Anti ERBB2(D769Y) Mouse Monoclonal Antibody-elisa试剂盒

产品详情

ERBB2(D769Y) [26382]
[100ul]

Catalog Number: 26382

Gene Symbol: ERBB2/HER2/Neu/c-Neu

Description: Anti ERBB2(D769Y) Mouse Monoclonal Antibody

Background: The HER-2/neu oncogene, a member of the epidermal growth factor receptor or erb-B gene-like family, encodes a transmembrane tyrosine kinase receptor that mediates extracellular signals activated by epidermal growth factors. Her2 abnormal has been strongly associated with many malignant tumors, especially with breast cancers. The expression level of Her2 is an important critiria in clinic evaluating of the progression of breast cancer.

Immunogen: A synthetic peptide from the internal region of ERBB2 which includes the mutation of D769Y, human origin.

Applications: ELISA, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
IF: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes D769Y mutant, but not wild-type ERBB2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Immunofluorescence:

Immunofluorescence of cells expressing ERBB2 proteins with Anti ERBB2(D769Y) antibody. HEK293T cells were transfected with pCDNA3-GFP ERBB2(D769Y) plasmid, pCDNA3-GFP ERBB2(WT) plasmid or pCDNA3-GFP ERBB2(D769H) plasmid, then fixed and stained with Anti ERBB2(D769Y) monoclonal antibody (Cat. #26382).

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