产品详情
人睾酮(T)ELISA试剂盒,48T/96T
产品有效期:六个月,有效截止期限,试剂盒上有标注
人睾酮(T)ELISA试剂盒,48T/96T,英文名称:T ELISA Kit,规格:96T/48T,常见别名:人睾酮(T)ELISA检测试剂盒,人睾酮(T)ELISA试剂盒,人睾酮(T)ELISA试剂盒,48T/96T
产品英文缩写:T ELISA Kit
ELISA试剂盒检测中样品的处理:
1)细胞培养物上清:细胞培养物于室温1000g,离心10分钟后收集上清,并将标本保存于-20℃,且应避免反复冻融。
2)血清:标本请于室温放置2小时或4℃过夜后于1000g,4℃离心20分钟,取上清即可检测,或将标本放于-20℃保存,但应避免反复冻融。
3)血浆:可用EDTA或肝素作为抗凝剂标本采集后30分钟内于2-8℃,1000g离心15分钟,或将标本放于-20℃保存,但应避免反复冻融。
4)尿液:无菌收集取初尿,离心除去沉淀物,即可用于检测,要长期保存尿样,可以加入0.05%的NaN3在4-8℃保存,或加入尿样冰冻保护液放入-20℃冰箱长期保存。
5)组织匀浆:将组织标本先用PBS洗涤,除去多余血液,匀浆化后放在PBS于-20℃放置过夜,再经过二次反复冻融破膜,5000g,4℃离心5分钟,取上清即可检测。
注:取样和样品处理过程中,防止有毒物质对操作人员的危害,要采取相应的保护措施。
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ELISA定性测定结果判定中常用的一些缩写:
(1)S/CO:其中S为sample(样本)或specimen(标本)的简写,表示的是标本测定的吸光度值,CO为cut-off值的简写。除竞争抑制法外,其他ELISA定性测定模式中,当S/CO值大于或等于1时,标本的测定为阳性,小于1时为阴性。
(2)S/N或P/N:其中S同(1),N为negative(阴性对照)的简写,P为patient(患者)的简写。较早的试剂盒很多都使用S/N或P/N≥2.1为阳性判定标准,现仍有一些人睾酮(T)ELISA试剂盒,48T/96T 使用这种方式。这种方式与S/CO方式无根本性区别,只不过是前者将阴性对照(N)的2.1倍视为cut-off值而已。
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