Polyethylenimine，Linear，Mw 25，000 线性化聚乙烯亚胺（PEI 25，000）

基本描述：

线性化聚乙烯亚胺PEI 25，000，一种高电荷阳离子聚合物，非常容易结合于高电荷阴离子基质。工业应用上，线性化PEI可改善负电荷染料的物理外观，以调整染料的化学特性和增强染料与固相基质的黏附能力，常用作黏附增强剂，作用同多聚赖氨酸（poly-lysine）；科研应用上，线性化PEI能与DNA或其他负电荷生物大分子简单结合，正是这一特性，使得成为一种非常行之有效的载体运输介质（Vector carrier），即转染试剂。

化学性质：

订购信息：【*，现货供应】

使用方法【来源于：CSH protocols，仅作交流学习】 ：

1. Pour ~450 mL of Milli-Q H₂O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH <2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

Approximay 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximay 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H₂O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.

应用文献【基因转染用途】 ：

Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.

Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pin (2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. Journal of Virological Methods. 138(1–2): 85–98.

Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy（2013）Transient Mammalian Cell Transfection with Polyethylenimine (PEI) . Methods Enzymol. 529: 227–240.

——Written/Edited by V. Shallan【版权归MKBio懋康所有】

线性聚乙烯亚胺（MW 25，000）